Observation techniques of nucleic acids and nucleoprotein complexes by AFM : Practical aspects of AFM DNA imaging

Practical aspects of AFM DNA imaging
Observation techniques of nucleic acids and nucleoprotein complexes by AFM

Authors : Loïc HAMON, Patrick A. curmi, David PASTRE

Review date: January 1, 2023 | Lire en français

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1. Practical aspects of AFM DNA imaging

Loïc HAMON is Senior Lecturer at the University of Evry.

Patrick A. CURMI is Director of Research, INSERM.

David PASTRE is Senior Lecturer at the University of Evry.

In the Structure-Activity of Normal and Pathological Biomolecules Laboratory, they work on characterizing protein/protein and DNA/protein interactions by AFM.

1.1 Operating principles of AFM imaging

The instrumentation and operating principles of the AFM have already been described in detail in this collection. However, for the sake of readability, it is necessary to recall the basic principles of AFM operation and the adaptation of the various imaging modes to the observation of...

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Practical aspects of AFM DNA imaging