Glossary
Experimental enzymology - From production to assays

new generation sequencing (NGS)

Molecular methodology for rapid sequencing. The libraries are prepared by random DNA fragmentation followed by hybridization of small, specific probes. Amplification is performed using increasingly sophisticated clonal amplification and PCR methods. Sequencing can be performed using a variety of approaches.

Chromatographie Liquide Haute Performance (CLHP); High Performance Liquid Chromatography (HPLC)

A technique for separating the constituents of a mixture. A pump injects the liquid(s) (mobile phase) under pressure onto a chromatography column (established stationary phase), which retains the various molecules to be separated from the mixture according to their physicochemical characteristics and the properties of the column used. To elute the components of the mixture, the system...