Conclusion
Main evolutions in liquid chromatography

Liquid chromatography is a technique that has evolved considerably since the early 2000s, with numerous technological innovations (stationary phases, HPLC instruments and analytical conditions) summarized in figure 14. The aim of these improvements is to respond to current problems, which are the need :

• the ability to carry out rapid/ultrarapid analyses, particularly in the pharmaceutical field;

• obtain very high resolutions for the analysis of the most complex mixtures (plant extracts, proteomics, human metabolomics, etc.);

• the availability of alternative separation methods offering...