Third-generation sequencing
Nucleic acid sequencing: methods, applications, developments and challenges

The third generation of mass sequencing is symbolized by Single Molecule Sequencing (SMS) .

Unlike the second generation, no DNA (or RNA) amplification is required for sequencing. A single molecule is read. This means that RNA can also be sequenced without first having to be converted into complementary DNA.

Several technologies and methods are under development, each with its own advantages and disadvantages. They can be grouped into three categories:

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