4. Quantification
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4.1.1 Isotopic metabolic labeling
The metabolic isotope labelling technique is based on the use of modified culture media incorporating labelled amino acids in place of native amino acids. As a result, proteins derived from cells grown on these modified culture media incorporate the tagged amino acids in their sequences. As a result, this technique limits its field of application to proteins from cultured cells. Isotope incorporation methodology was first presented in 2002 by Matthias...
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Quantification
Bibliography
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(1) - KAHN (P.) -
Science, 20, p. 369-370 (1995).
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(2) - WILKINS (M.R.), SANCHEZ (J.C.), GOOLEY (A.A.), APPEL (R.D.), HUMPHERY-SMITH (I.), HOCHSTRASSER (D.F.), WILLIAMS (K.L.) -
Biotechnology and Genetic Engineering Reviews, 13, p 19-50 (1995).
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