Fluorescent preparations
Biomedical fluorescence microscopy

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BIO7200 V1 Article

Fluorescent preparations
Biomedical fluorescence microscopy

Authors : Léon ESPINOSA, Yves TOURNEUR

Publication date: March 10, 2015 | Lire en français

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1. Fluorescent preparations

1.1 The physical principle of molecular fluorescence

Light interacts with a fluorescent molecule by absorbing a photon, which transfers its energy to a peripheral electron. This electron then restores part of the acquired energy in the form of light radiation. These are the well-known phenomena of fluorescence and phosphorescence, described in the literature ([P 2 835] and [P 2 655]).

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