Glossary – Definitions
Microfluidics and Tumor DNA - Contribution of digital PCR

DNA :

Deoxyribonucleic acid.

RNA :

Ribonucleic acid.

PCR (Polymerase Chain Reaction) :

Polymer chain reaction.

Sanger sequencing :

Chain-termination" sequencing technique.

NGS(Next Generation Sequencing) :

Next-generation sequencing.

qPCR(quantitative PCR or real time PCR) :

Quantitative or real-time PCR.

PDMS (poly(dimethylsiloxane)) :

Transparent polymer used to manufacture microfluidic chips.

Dilution limit :

Dilution calculated so as to have only one object (DNA molecule, cell, etc.) per compartment.

Monodisperse :

Characterizes particles of similar size (in an emulsion or colloid).

Fluorescent probes :

FAM™: 6-carboxyfluoroscein; max Ex 494 nm – max EM 517 nm.

NED™: chemical structure not available (proprietary to Life Technologies); max Ex 553 nm – max...