From PCR to digital PCR
Microfluidics and Tumor DNA - Contribution of digital PCR

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RE241 V1 Research and innovation

From PCR to digital PCR
Microfluidics and Tumor DNA - Contribution of digital PCR

Authors : Philippe NIZARD, Aurélie KROL, Pierre LAURENT-PUIG, Valérie TALY

Publication date: February 10, 2015 | Lire en français

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3. From PCR to digital PCR

3.1 Polymerase chain reaction (PCR)

PCR has become the most widely used technique for detecting nucleic acid sequences. It involves the specific amplification of a known DNA sequence from a small number of starting copies, using specific primers and the enzyme polymerase. The known sequence is then amplified exponentially (figure 2 ).

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