6. Glossary
Field of view
Total surface area that can be seen through the lens and imaged on the camera sensor.
Working distance
Distance between object and lens when image is sharpest.
Quenching
All the phenomena that contribute to reducing the fluorescence intensity of a given substance.
Magnification
Ratio of sensor size to field of view size.
Photobleaching
Irreversible loss of fluorescence of a molecule.
Depth of field
Distance range within which the object appears to be in acceptable focus.
Resolution
Minimum distance between two points that can still be considered distinct....
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