Glossary – Definitions
Biomedical fluorescence microscopy

Glossary and reference source for the techniques described in this article and in recent literature in May 2014.

4 PI

4π solid angle imaging, in which the preparation is inserted between two objectives used simultaneously for two-photon excitation and image collection doi:10.1364/JOSAA.9.002159

BALM (Bleaching/Blinking Assisted Localization Microscopy)

Super-resolution microscopy identifying single fluorescent molecule spontaneous ignition and extinction events by difference between successive frames doi:10.1073/pnas.117430109

BiFC (Bimolecular Fluorescence Complementation )

Identification of a reaction by binding one fluorophore half to each of two components doi:10.1146/annurev.biophys.37.032807.125842