7. Quantitative analysis
Once the solute(s) of interest have been identified, the chromatogram can be used for quantitative analysis using the relationship :mi = KiA i
which relates the mass mi of the solute i injected to the area of the peak Airepresenting this solute, due to the Gaussian shape of the differential curves traced on the recorder.
It is therefore necessary to measure the areas of the peaks and to determine, for each solute, the proportionality coefficient Ki.
7.1 Measuring peak area
The area of the peak is defined as the portion of the surface between the curve itself and the extension of the base line.
The classic methods for measuring it are as follows.
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Triangulation: the peak...
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Quantitative analysis
Sample preparation
This "For further information" section contains the following tables:
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