Conclusion
Next generation sequencing - Tools and issues for human health

Next-generation sequencing techniques have now become widely accessible. The range of sequencers has expanded and currently offers a wide variety of machines suited to different settings (research laboratories, hospitals, etc.) and specific applications. Thanks to short-read sequencers, it is possible to generate a very large number of sequences in record time and at a very low cost. In less than twenty years, the cost of sequencing a human genome has dropped to an affordable $100. However, these machines have certain limitations, such as the generation of relatively short sequences (fewer than 1,000 bases), difficulties in sequencing regions rich in GC or homopolymers, and biases associated with PCR amplification of the sample. To address this, companies have developed devices capable of sequencing long fragments without amplifying the initial sample. However, the error rate remains higher than...