1. Fat extraction and dosing
The purpose of fat extraction is twofold:
It must therefore be total and not alter the nature of the fat.
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1.1.1 Direct solvent extraction
In some cases, for wet products, it is necessary to remove the aqueous phase prior to extraction, either by oven drying or by drying on anhydrous sodium sulfate Na2SO4.
The solvents or solvent mixtures most often used are :
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Fat extraction and dosing
Bibliography
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(1) - FOLCH (J.), LESS (M.), SLOANE-STANLEY (G.M.) - A simple method for the isolation and purification of total lipids from animal tissues. - J. Biol. Chem., 226, p. 497-509 (1957).
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(2) - BLIGH (E.G.), DYER (W.J.) - A rapid method for total lipid extraction...
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