Glossary
Next generation sequencing - Tools and issues for human health

Adaptor; Adaptateur

Short DNA sequences for sequencing primer attachment.

Amplicon; Amplicon

DNA fragment amplified by PCR.

RNA Spike-In

RNA transcript of known sequence and quantity used as internal calibrator.

Bar code; Barcode

Small DNA fragment of a few bases used to identify libraries.

Dysbiosis; Dysbiosis

Unbalanced bacterial ecosystem, particularly in the intestinal tract.

Exome; Exome

Set of genome exons coding for proteins.

Flow cell

Solid support on which sequencing is performed.

Fusion gene; Fusion gene

Hybrid gene formed by two distinct genes.

Homopolymer; Homopolymer

Sequence of several identical bases (AAAAAAA, GGGGGG).

Identificateur moléculaire unique; Unique molecular identifiers (UMI)

Barcodes used in single-cell RNAseq.

Isoform; Isoform