4. OECD Test No. 429: Skin Sensitization Test: Local Lymph Node Assay (LLNA) in Mice
The LLNA test is based on the principle that sensitizers induce lymphocyte proliferation in the lymph nodes draining the site of application of the test substance. This proliferation is proportional to the dose applied and the potency of the allergen. It provides an easy way to obtain a quantitative measure of sensitization.
4.1 Induction
Days 1, 2, and 3: Apply 25 μl of an appropriate dilution of the test substance, vehicle alone, or TP to the back of each ear.
4.2 Latency
On days 4 and 5, no treatment is applied.
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OECD Test No. 429: Skin Sensitization Test: Local Lymph Node Assay (LLNA) in Mice
OECD Test No. 442A: Skin sensitization. Local lymph node stimulation test (DA)
Bibliography
A. Lombard et al. - Technical and economic evaluation of products in the early stages of development. Part 2, pp. 153-281, ISTE Publishing Ltd London (2024).
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The European Chemicals Agency implements EU chemicals legislation to protect human health and the environment. Its work also contributes to the smooth functioning of the internal market, innovation, and the competitiveness of the European chemical industry. Everything is made from chemicals. Improving knowledge of hazardous products...
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