Human test: Standard Test Method for Human Repeat Insult Patch Testing(HRIPT), ASTM D6355
Detecting sensitizing substances through animal and human testing

This test was developed in the USA and standardized by ASTM to assess the sensitizing potential of a cosmetic preparation or medical device intended to remain in contact with the body.

The test principle is the same as for animal testing:

• induction: study material is applied at relatively low or non-irritating doses to the volunteer's back or arm for three weeks;

• two-week latency period;

• Triggering: a single application of a non-irritating dose is made for 48 hours;

• skin reaction scoring: erythema and edema are examined and scored.