Conclusion and outlook
Highly sensitive biological detection test by means of new fluorescent tracers

The use of liposomes as a new fluorescent tracer in strip tests and immunoassays paves the way for other solid-phase applications in the future. Among these applications, the ELISpot technique (solid support corresponding to a nitrocellulose or PVDF membrane) seems highly appropriate, as do DNA or protein chips (solid support in a glass slide).

The ELISpot is an analytical test for counting cells according to their secretion. The principle involves capturing molecules secreted by cells on a solid support using specific antibodies. After removal of the cells, the immunocomplex is revealed by a peroxidase-coupled antibody, using an insoluble chromogenic substrate whose localized precipitation generates colored spots or immunospots. The use of immunoliposomes in this format may prove interesting, as these tracers would enable faster results to be obtained (without...