Some methods for assessing the impact of plant protein expression on lipid bodies and lipid content
Optimizing the genetic production of lipids through yeasts by means of genetic engineering processes

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Some methods for assessing the impact of plant protein expression on lipid bodies and lipid content
Optimizing the genetic production of lipids through yeasts by means of genetic engineering processes

Authors : Céline BOULARD, Marine FROISSARD

Publication date: February 10, 2012 | Lire en français

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7. Some methods for assessing the impact of plant protein expression on lipid bodies and lipid content

7.1 Morphological characterization of lipid bodies

The changes in lipid bodies induced following expression of caléosine in Saccharomyces cerevisiae are assessed by two complementary techniques: an in situ approach, using electron microscopy, and a post-isolation approach, using laser-assisted dynamic light scattering.

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7.1.1 Assessment of the number and size of lipid bodies in situ by transmission electron microscopy

Cells expressing AtClo1-GFP are prepared (fixation, embedding and sectioning)...

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Some methods for assessing the impact of plant protein expression on lipid bodies and lipid content

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