4. Carbohydrate assay methods
The first problem that arises when studying a free or conjugated oligo- or polysaccharide concerns its monosaccharide composition. This raises two questions:
1) What is the compound's centesimal composition of neutral monosaccharides, osamines, uronic acids and sialic acids?
2) What is its molar composition in monosaccharides divided into the four above families?
4.1 Determination of centesimal monosaccharide composition by colorimetric methods
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General rules
1. Temperature and heating time are of great importance in the development of stains, and must be precisely defined.
2. All test tubes used must have the same diameter and be rigorously clean.
3. Before being placed in the water bath, the reaction media are carefully homogenized...
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Carbohydrate assay methods
Determining the primary structure
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