The fluidic equilibrium established between the stationary and mobile phases throughout the column results in a pressure drop ΔP, which includes a hydrostatic term related to the density difference (density difference) and the centrifugal field, and a hydrodynamic term related to the viscosity of the mobile phase and the flow rate:
( 6 )
with :
ΔP
:
head loss generated by the column,
...
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(1) - MARTIN (A.J.P.), SYNGE (R.L.M.) -
A new form of chromatogram employing two liquid phases : a theory of chromatography. 2. Application to the micro-determination of the higher monoamino-acids in proteins.
-
Biochemical Journal, 35, p. 1358-1368 (1941).
(2)...
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