Quantitative analysis
Liquid chromatography - Separation theory and methods

To obtain reproducible, meaningful results, you need both perfectly stable operating conditions and a sample that is representative of the analysis problem at hand.

Most of the detectors (absorptiometric, fluorimetric, electrochemical, etc.) used in LC are differential detectors that measure the variation of quantities proportional to the concentration of the solute in the effluent; peaks are obtained whose area is linked to the quantity of solute that has passed through the detector by the relationship :