Formation of labile diastereomers in the mobile phase
Chiral separations by LC, SPC and GPC

The addition of a chiral derivatization reagent to the mobile phase is mainly used in liquid chromatography. In gas chromatography, the solvent power of the mobile phase is too low (however, we should mention the study by Maestas et al. [14] on the resolution of racemic bicyclic alcohols and ketones by GPC on achiral stationary phases by adding a volatile chiral derivatization reagent (d-limonene, l-menthol, etc.) to the injection, resulting in in situ derivatization). Two chiral discrimination mechanisms are conceivable. Labile diastereoisomers can be formed either within the chiral mobile phase, or on the surface of the stationary phase after impregnation with the chiral reagent. It is likely that these two mechanisms coexist.