3. From PCR to digital PCR
PCR has become the most widely used technique for detecting nucleic acid sequences. It involves the specific amplification of a known DNA sequence from a small number of starting copies, using specific primers and the enzyme polymerase. The known sequence is then amplified exponentially (figure 2).
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From PCR to digital PCR
Bibliography
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(1) - KRZYSZTOF (C.), TOMASZ (S.K.), SLAWOMIR (J.) et al - Rapid screening of antibiotic toxicity in an automated microdroplet system. - Lab. Chip., 12, p. 1629-1637 (2012).
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(2) - BROUZES (E.), MEDKOVA (M.), SAVENELLI (N.) et al - Droplet microfluidic technology for...
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