Photo-Patch test in humans
Detecting sensitizing substances through animal and human testing

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FIC1263 V2 Practical sheet

Photo-Patch test in humans


Detecting sensitizing substances through animal and human testing

Author : Alain LOMBARD

Publication date: November 10, 2025 | Lire en français

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10. Photo-Patch test in humans

This test was developed to confirm the absence of photosensitizing potential in a substance or mixture after repeated application under a bandage and exposure to UV light.

The study lasts six to eight weeks. This test is carried out under the supervision of a dermatologist.

The test is not validated by the OECD, but it is often used in cosmetics as a replacement for animal testing.

10.1 Induction

The product is applied under an occlusive or semi-occlusive dressing (depending on its composition) for 24 hours on the volunteers' backs, twice a week for three consecutive weeks.

Exposure to UV rays at a slightly erythematogenic dose is performed after each dressing change.

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