3. Buehler method: skin applications only (OECD test no. 406: skin sensitization)
3.1 Induction
Day 0 – treated group: one flank is hair-free (shorn short). The test patch must be completely soaked with test substance in a suitable vehicle (the choice of vehicle must be justified; liquid test substances can be applied undiluted if necessary). The test patch is applied to the test area and maintained in contact with the skin for 6 hours using an occlusive dressing or a cup and suitable dressing. A dry patch is applied to the flank of animals in the control group. The test patch must be occlusive, measuring approximately 4 to 6 cm 2 .
Days 6-8 and 13-15 – treated and control groups: between days 6 and 8 and again between days 13 and 15, the same application as on day 0 is applied to the same test...
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Buehler method: skin applications only (OECD test no. 406: skin sensitization)
Skin sensitization test: Local lymph node assay in mice, LLNA (Local Lymph Node Assay), OECD no. 429
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www.oecd.org OECD Guidelines for the Testing of Chemicals: these are a set of internationally-agreed test methods used by governments, industry and independent laboratories to determine the safety of chemicals and formulations, including pesticides and industrial chemicals. They cover tests on the physico-chemical properties of substances, their effects on human...
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