4. Conclusions and outlook

The disadvantages of first-generation superficially porous stationary phases for the analysis of small molecules, in terms of loading capacity and retention, have been overcome with the appearance since 2006 of $\mathrm{sub}\text{-}3\mathrm{\mu m}$ phases with sufficiently thick porous layers. As a result, the use of these supports is no longer limited to the analysis of large biomolecules, but is applicable to all types of molecules, as well as to all modes of liquid and supercritical phase separation. New stationary phase chemistries are continually appearing on the market, as well as different morphologies adapted to given separations. Work is underway...