Article | REF: P1457 V1

Two-dimensional liquid chromatography (2D-LC)

Authors: Sabine HEINISCH, Morgan SARRUT

Publication date: August 10, 2020 | Lire en français

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6. Glossary

Chromatographie liquide; liquid chromatography

A technique for separating molecules according to their relative affinity between a retentive stationary phase and an eluting mobile phase.

Orthogonality

Two chromatographic systems are said to be orthogonal for a given group of compounds when the retentions in these two systems are not correlated.

Gradient time

Time (programmed on the device) to go from an initial composition to a final mobile phase composition during an elution gradient.

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Bibliography

(1) - GROSKREUTZ (S.R.), SWENSON (M.M.), SECOR (L.B.), STOLL (D.R.) - Selective comprehensive multidimensional separation for resolution enhancement in high performance liquid chromatography. - Part II : Applications, Journal of Chromatography A (2011)