Predicting recovered concentrations by inverse calibration
The accuracy profile method

Before calculating the validation parameters, the instrumental responses measured on the samples (arbitrary unit) must be transformed into concentrations (mg L -¹ ). This is done by taking the inverse function of the calibration model.

• Note Y the instrumental response and X the concentration of the calibration solution. The calibration model is a straight line whose coefficients a ₀ and a ₁ can be estimated by the least-squares linear regression method : $Y=a_0+a_{1}X+\text{ε}$ .

• Once these coefficients a ₀ and a ₁ are known, the inverse calibration function used to predict the recovered concentration of...