1. Nucleic acid preparation techniques
A large number of methods are used to extract nucleic acids. The choice of one or other of these techniques will depend on the cell type used (mammalian cells, lower eukaryotes, plants, bacteria, viruses...), the starting material (whole organ, tissue, cell culture, blood...), the expected result (purity, preparation time...) and the applications envisaged (PCR, RT-PCR, cloning, probe labelling, Southern, Northern blot, RNase protection...).
1.1 DNA
There are numerous methods for extracting DNA: saline extraction, affinity columns, leukocyte lysates
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Nucleic acid preparation techniques
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