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Article | REF: MED500 V1

3D visualization of the cerebral vasculature by two-photon microscopy

Authors: Florence APPAIX, Cyrille MONNEREAU, Boudewijn VAN DER SANDEN

Publication date: August 10, 2014 | Lire en français

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    3. Two-photon fluorescent probes for intravital and cerebrovascular imaging

    3.1 Two-photon excitable fluorescent probes

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    3.1.1 General background/spectroscopic criteria

    Like most modern biomedical imaging techniques, two-photon optical microscopy requires the use of molecular contrast agents, in this case fluorescent probes capable of efficiently absorbing two photons simultaneously. The mechanisms of nonlinear optics in general, and of multiphoton absorption processes in particular, correspond to complex physical processes whose theoretical underpinnings go far beyond the scope of this article....

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    Two-photon fluorescent probes for intravital and cerebrovascular imaging
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