4. Advanced microscopy techniques

4.1 Restricting the area explored

In this section, we will describe specific microscopy techniques for limiting the size of the "blur" zone created by a fluorescent source. The causes of blur are mainly due to the illumination of fluorescent objects outside the plane of focus and to the image spreading function of a point source ("PSF"). The various confocal microscopes and TIRF described below improve the first situation, while super-resolution techniques "restrict" the PSF. These restrictions improve...