Isothermal amplification processes
Molecular techniques for detection of foodborne bacteria
Quizzed article REF: RE281 V1
Isothermal amplification processes
Molecular techniques for detection of foodborne bacteria

Authors : Jasmina VIDIC, Sandrine AUGER

Publication date: December 10, 2019, Review date: January 29, 2021 | Lire en français

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3. Isothermal amplification processes

Due to the need to heat samples during PCR cycles, conventional PCR-based methods are not suitable for the development of portable point-of-care diagnostic kits. This problem is solved by isothermal amplification strategies, which are performed at a constant temperature. These methods share with PCR the specific amplification of one or more molecular markers (DNA) by a DNA polymerase. Isothermal amplification covers a wide range of methods

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