Applications
AFM techniques for observing nucleic acids and nucleoprotein complexes
Article REF: RE115 V1
Applications
AFM techniques for observing nucleic acids and nucleoprotein complexes

Authors : Loïc HAMON, Patrick A. curmi, David PASTRE

Publication date: September 10, 2008 | Lire en français

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3. Applications

3.1 Nucleic acid imaging

High-resolution imaging is required to observe and study the conformation or structural changes of nucleic acids in isolation or in interaction with partners. In the case of double-stranded DNA, the persistence length in salt concentrations close to those found in physiological media is of the order of 50 nm, enabling this molecule to be particularly extended on the surface (figure 5 ) and therefore to have a well-defined contour.

RNA: ribonucleic acid

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