Article | REF: J2786 V1

Preparative electrophoresis

Authors: Hélène ROUX de BALMANN, Victor SANCHEZ

Publication date: March 10, 1997 | Lire en français

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    4. Processes designed with preparation in mind

    4.1 General operating principles

    To enable the separation of larger quantities of products, it was necessary to develop processes of a different design to those existing on the analytical scale. The idea was to carry out electrophoresis in a flowing liquid. In addition to increasing the quantities processed, these processes enable products to be recovered directly from the liquid phase, without the need for elution techniques. Several devices have been proposed, operating either in continuous or discontinuous mode, and sharing certain common features. They all feature refrigeration systems of varying degrees of sophistication. In addition, the electrodes are placed in compartments separated from the electrophoresis chamber by membranes, to prevent electrolysis gases from interfering...

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