3. Contrast amplification in light microscopy
Research into contrast amplification has also touched on more traditional techniques such as optical microscopy. Developments initially focused on optical signal modulation (DIC technique), then moved on to the field of contrast-amplifying media.
3.1 DIC technique (Differential Interference Contrast)
The two main functions of an optical microscope are to increase the size and maximize the resolution of an image to make a microscopic specimen visible. However, the human eye or detectors are sensitive to variations in intensity or color and in many cases, for example in living media, contrast is so poor that specimens remain invisible regardless of optical resolution. The same applies to objects that do not absorb light, often referred to as "phase objects"....
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Contrast amplification in light microscopy
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