2. How do you establish a TRV?

The first step is to establish the toxicological profile of the substance and collect all relevant data, then select the most relevant and valid studies (Klimisch).

Physico-chemical data relevant to inhalation must also be collected, in particular form (gas or particles) and volatility (boiling point and vapor pressure – PKa) as well as solubility in aqueous and lipid media (partition coefficient – log K O/W).

Next, we need to determine the substance's modes of action (MoA) and identify the effects observed on cells and organs (threshold and non-threshold effects).

Finally, it is necessary to...