Article | REF: P3255 V2

Functional analysis by colorimetry and fluorimetry

Authors: Gwenola BURGOT, Fernand PELLERIN

Publication date: March 10, 2005, Review date: October 1, 2017 | Lire en français

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4. Conclusion

The use of color reactions, once reserved for the identification of organic molecules, has spread to other fields. What's more, technology has evolved in recent years to facilitate the implementation of these color reactions, with the use of optical fibers for remote spectrophotometric measurements. These systems are ideal for on-site control of raw materials, working in explosive atmospheres or highly contaminated environments, or for on-line monitoring of manufacturing processes.

Similarly, in the field of bioanalysis, the evolution of technology has enabled new applications such as the coupling of a fluorescence marker or fluorophore to nucleic acid molecules to produce biological probes. These are used in genetic cytology laboratories to detect biochemical anomalies responsible for malformations or cancer. Fluorescence markers are small molecules that undergo...

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