1. Make sure there's no contamination
This is the effect exerted by a sample on the one that follows or precedes it (memory effect). After cleaning, a concentrated sample is analyzed three times consecutively ( H 1, H 2 and H 3, average mH ), then a low-concentration sample is also analyzed three times ( B 1, B 2 and B 3). This series of measurements is repeated three to five times. The mean values mB 1, mB 3 and mH are then calculated. The percentage of contamination is then calculated as follows:
Contamination (%) = ( mB 1 - mB 3) * 100
mH - mB 3
This memory effect should be close to zero %. This is how we test the cleaning between two analyses.
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Make sure there's no contamination
Bibliography
Analytical validation guide: SFSTP commission report: 1994 and 2006
Guideline ICHQ2 (R1), Validation of Analytical Procedures: text and methodoly
NF T 90-210, Protocole d'évaluation initiale des performances d'une méthode dans un laboratoire – AFNOR – 2009
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