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Quizzed article | REF: P3365 V2

Capillary electrophoresis Principles

Author: Nathalie DELAUNAY

Publication date: December 10, 2019, Review date: September 2, 2020 | Lire en français

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    1. General principle and transport phenomena

    1.1 Principle

    Capillary electrophoresis is a technique in which compounds are analyzed within a capillary of small internal diameter (a few tens of micrometers) and a length of the order of a few tens of centimeters, filled with a separating electrolyte. The two ends of the capillary dip into two different reservoir bottles, each containing two electrodes connected to a high-voltage generator, as shown schematically in figure 1 . Applying a voltage of up to several tens of kV to the ends of the capillary generates a longitudinal electric field within the capillary, which sets the analytes in motion at their own speed. A continuous detection step is performed, usually...

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