2. Diastereomer formation by precolumn derivatization
This method involves pre-reacting the racemate with an optically pure reagent to form diastereoisomers; these have different physico-chemical properties and can therefore be separated using conventional stationary and mobile phases. This method is limited to molecules with reactive functions (amines, acids, alcohols, etc.). For preparative separations, the return to the initial enantiomer may be accompanied by partial racemization, which is another limitation of this method.
2.1 Gas chromatography
As early as 1960, enantiomer separations were performed after prior derivatization into diastereoisomers. See
Exclusive to subscribers. 97% yet to be discovered!
Already subscribed? Log in!
Diastereomer formation by precolumn derivatization
Formation of labile diastereomers in the mobile phase
Article included in this offer
"Analysis and Characterization"
(
271 articles
)
Updated and enriched with articles validated by our scientific committees
A set of exclusive tools to complement the resources
References
Exclusive to subscribers. 97% yet to be discovered!
Already subscribed? Log in!
