Article | REF: P1455 V2

Liquid chromatography - Separation theory and methods

Authors: Marcel CAUDE, Alain JARDY

Publication date: October 10, 1994, Review date: February 5, 2019 | Lire en français

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    A  |  A

    2. Kinetics

    2.1 Reduced sizes

    In plateau theory, the HEPT deduced from the variance σ 2 of the elution peak : H=LN=Lσ2tR2

    can only be seen as a global measure of the influence of all the experimental parameters involved, but it doesn't allow us to specify the contribution of each parameter to the spreading of the peak as it progresses through the column. Spread depends on :

    • the diameter and nature of the particles...

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