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Article | REF: P1473 V1

Planar chromatography - Part 1

Authors: Antoine Michel SIOUFFI, Chantal DAUPHIN, Dominique PRADEAU

Publication date: March 10, 2007, Review date: November 1, 2022 | Lire en français

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    Overview

    ABSTRACT

    This article is mainly dedicated to thin layer (or planar) chromatography. A true economic alternative, this reliable method has applications in domains as varied as legal medicine, pharmaceutics or the food industry. The chromatographic process and its implementation are firstly presented: comparisons, application domains, etc. Fundamental quantities in planar chromatography are then dealt with for a better understanding of the phenomenon. To conclude, a detailed analysis of the stationary/mobile phase combinations (and retention mechanisms) is provided.

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    AUTHORS

    • Antoine Michel SIOUFFI: Doctor of Science - Professor Emeritus, Paul Cézanne University, Aix Marseille III

    • Chantal DAUPHIN: Doctor of Pharmaceutical Sciences - Senior lecturer at Paris XI University

    • Dominique PRADEAU: Doctor of Pharmaceutical Sciences - Pharmacist in charge of the Établissement pharmaceutique des Hôpitaux de Paris

     INTRODUCTION

    Advances in adsorbent materials, instrumentation, quantification and detection capabilities have led to a renaissance in planar chromatography. Thin layer chromatography (TLC) is not only a cost-effective alternative, but also a practical, rapid and reliable method that does not require highly complex sample preparation.

    It is used for screening in clinical toxicology, forensic medicine and pharmacognosy.

    It plays an important role in the control of pharmaceutical products (identification, purity, stability, dosage) and food products (detection of toxic impurities).

     

    Main abbreviations

    CCM

    Thin layer chromatography

    CLHP

    Liquid chromatography on high-performance columns

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