Planar chromatography -Part 2

In conventional planar chromatography, substance detection is a separate operation from chromatography, and takes place on a layer freed from the developing solvent: this is called batch detection. Forced-flow planar chromatography can be used in the same way, but it is also possible to achieve continuous detection using a detector similar to those used in HPLC. In batch detection, the general approach is to compare regions of the layer where no sample is present with regions where sample is present. When enough substance can be recovered for spectral identification, the plate is scratched where the substance has been located, the stationary phase is separated from the solute, and all that remains is to perform spectrometry (infrared, mass, etc.). We'll now compare the different analysis methods, both qualitatively and quantitatively.