Overview
FrançaisABSTRACT
Capillary electrophoresis is a separation technique, often marketed in the form of instruments whose stages, including sample injection, separation and detection, generally optical, are fully integrated. The attraction of the latest miniaturized version is notably due to the prospect of very rapid analysis and development of labs on chips. This scaling is not without consequence, in particular on injection and detection stages, which use other technologies.
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Read the articleAUTHORS
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Agnès HAGÈGE: CNRS Research Associate at the Institute of Environmental Biology and Biotechnology, UMR 7265 CNRS-CEA-Aix-Marseille II and SBTN, CEA Marcoule
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Thi Ngoc Suong HUYNH: Graduate of the Faculty of Pharmacy, Ho Chi Minh City (Vietnam) Doctoral student in the Biochemistry and Nuclear Toxicology Department, CEA Marcoule - This article is an updated version of [P 3 366], published in 2003 by Myriam TAVERNA, Isabelle LE POTIER and Philippe MORIN.
INTRODUCTION
Capillary electrophoresis is a separative technique based on the migration of charged species in an electric field, according to their electrophoretic mobility.
It requires only very small quantities of sample and offers the advantage of high separation efficiency, low operating costs and low waste generation.
Today, it is recognized as a separative technique in its own right, and is integrated into numerous analytical platforms in the pharmaceutical, biomedical and environmental fields. It is applicable to the separation of a wide variety of compounds, from small inorganic ions to biological macromolecules (sugars, proteins, DNA, etc.).
An important part of its implementation is due to its relatively simple equipment, with few moving parts, making it suitable for miniaturization. Thanks to these features, its integration into lab-on-a-chip systems has generated considerable interest in the field of analysis.
In addition, ongoing instrumental developments have enhanced its performance and potential. In this respect, coupling with mass spectrometry can be seen as a decisive step forward in the expansion of this technique.
The aim of this article is to give an overview of the equipment used in capillary electrophoresis, detailing its main components and mentioning the most frequently used configurations and the many developments, particularly in the field of detection. An important part is devoted to the miniaturization of this technique.
This article is part of a series dedicated to capillary electrophoresis:
"Capillary electrophoresis. Applications "
.[P 3 367]
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KEYWORDS
state of art | instrumentation | environment | analytical chemistry | | | | |
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Capillary electrophoresis
Bibliography
Directory
Manufacturers – Suppliers – Distributors (non-exhaustive list)
Agilent Technologies (conventional equipment) http://www.chem.agilent.com
Applied Biosystems (conventional equipment) http://www.appliedbiosystems.com
Beckman...
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