1. Home
  2. Measurements - Analysis
  3. Laboratory quality and safety procedures
  4. The accuracy profile method

  5. Define the scope of the method to be validated and quantify the objectives

Practical sheet | REF: FIC1420 V1

The accuracy profile method

Author: Max FEINBERG

Publication date: April 10, 2015 | Lire en français

You do not have access to this resource.
Click here to request your free trial access!

Already subscribed? Log in!

Automatically translated using artificial intelligence technology (Note that only the original version is binding) > find out more.

    A  |  A

    2. Define the scope of the method to be validated and quantify the objectives

    As an illustration, we will use the validation study of a method for determining nicotinamide (a vitamin B 3 vitamin) in milk by HPLC, with quantification by external calibration.

    • The natural content of nicotinamide in milk is between 0.6 and 1.0 mg L - 1 ; regulations authorize supplementation, ranging from 0.4 to 2.0 mg L - 1 . The chosen validation range is therefore between 0.4 and 4.0 mg L - 1 .

    • There is no official reference for setting the acceptability limits of this method. However, the microbiological reference method has a high uncertainty of ± 40%. To demonstrate that the HPLC method can easily be an alternative to the reference method, acceptability limits...

    You do not have access to this resource.

    Exclusive to subscribers. 97% yet to be discovered!

    You do not have access to this resource.
    Click here to request your free trial access!

    Already subscribed? Log in!

    The Ultimate Scientific and Technical Reference

    A Comprehensive Knowledge Base, with over 1,200 authors and 100 scientific advisors
    + More than 10,000 articles and 1,000 how-to sheets, over 800 new or updated articles every year
    From design to prototyping, right through to industrialization, the reference for securing the development of your industrial projects

    This article is included in

    Laboratory quality and safety procedures

    This offer includes:

    Knowledge Base

    Updated and enriched with articles validated by our scientific committees

    Services

    A set of exclusive tools to complement the resources

    Practical Path

    Operational and didactic, to guarantee the acquisition of transversal skills

    Doc & Quiz

    Interactive articles with quizzes, for constructive reading

    Subscribe now!

    Ongoing reading
    Define the scope of the method to be validated and quantify the objectives
    Outline