6. Predicting recovered concentrations by inverse calibration
Before calculating the validation parameters, the instrumental responses measured on the samples (arbitrary unit) must be transformed into concentrations (mg L - 1 ). This is done by taking the inverse function of the calibration model.
Note Y the instrumental response and X the concentration of the calibration solution. The calibration model is a straight line whose coefficients a 0 and a 1 can be estimated by the least-squares linear regression method : .
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Predicting recovered concentrations by inverse calibration
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Bibliography
Collège Français de Métrologie: Application of the new VIM3 calibration concept (Reissued 2014)
Commission Decision of August 12, 2002 implementing Council Directive 96/23/EC as regards the performance of analytical methods and the interpretation of results (2002/657/EC)
Feinberg (M.), Labo-Stat: Guide de validation des méthodes d'analyse...
Normative references
ISO 16140: 2003 Microbiology of food: validation protocol for alternative methods (2003) ISO, Geneva
NF EN ISO/CEI 17025 : 2005 General requirements for the competence of testing and calibration laboratories (2005) ISO, Geneva
NF V03-110 (May 2010) Analysis of agricultural and food products – Characterization protocol for the validation of a quantitative analysis method...
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