1. Chromatographic process
1.1 Comparison of column and planar liquid chromatography
The most important part of a chromatograph is the stationary phase/mobile phase assembly. Every chromatographic operation involves :
moving the mobile phase;
injection of solutes ;
the chromatographic process ;
solute detection.
In high-performance liquid chromatography (HPLC), the mobile phase is pumped into the column, with the operator in control of the flow rate. Injection is performed by a loop valve without interrupting the pump flow, and the solutes are injected into a balanced system. The chromatographic process takes place but is not...
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Chromatographic process
Implementation of planar chromatography
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