1. Principles
1.1 Absorption of radiation in the UV-VIS range
The principle of ultraviolet and visible absorption spectrometry is based on the absorption of radiation by molecules in the range from 190 to 800 nm, which corresponds to the ultraviolet (190-400 nm) and visible (400-800 nm) ranges. Some spectrophotometers also cover the near infrared up to 2,500 nm, for example. This range is illustrated in Figure 1 .
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Principles
Instrumentation
Bibliography
▪ Publications cited in the text
Standards
French Standardization Association AFNOR:
• Guide to the expression of measurement uncertainties. NF ENV 13005 (1999).
• Molecular Absorption Spectrophotometry – Vocabulary and General Information. T01-030 (December 1982).
• Analytical chemistry – Volume 1: analysis, fundamental standards – Volume 2: sampling, general methods of analysis and reagents, ISBN: 2-12-172911...
Regulations
In the field of UV-VIS molecular absorption spectrophotometry, there are many organizations that publish regulations or recommendations:
either in specific fields: US and EU Pharmacopeia for pharmacy;
either in general fields: ASTM, LNE, Eurachem, etc. for analysis methods and data processing, IUPAC for vocabulary;
...
Manufacturers – Suppliers – Distributors
(Non-exhaustive list)
The contact details of UV-VIS spectrophotometer manufacturers can be found on specialized websites such as Globalspec http://www.globalspec.com or through search engines. This information is listed in the section below (please note that European manufacturers...
Websites
While the Internet is an extraordinary source of information, finding the right information is not always easy, particularly in scientific and technical fields. Information is generally searched for using search engines or meta search engines that aggregate results from several engines.
Two difficulties arise in this research:
the choice of keywords to launch...
Organizations
The contact details for learned societies and organizations involved in spectrophotometry are provided in the "Websites" section above. These organizations often publish free or paid journals and have a document library that can be accessed from their website.
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