3. OECD Test No. 406: Skin sensitization, Buehler method: skin applications only
3.1 Induction
Day 0 – test group: one flank is shaved (close shave). The test patch must be completely saturated with the test substance in a suitable vehicle (the choice of vehicle must be justified; liquid test substances may be applied undiluted if necessary). The test patch is applied to the test area and is kept in contact with the skin for 6 hours using an occlusive dressing or a cup and an appropriate dressing. A dry patch is applied to the flank of the animals in the control group. The test patch should be occlusive and should measure approximately 4 to 6 cm 2 .
Days 6-8 and 13-15– d treatment group and control group: between days 6 and 8 and again between days 13 and 15,...
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OECD Test No. 406: Skin sensitization, Buehler method: skin applications only
OECD Test No. 429: Skin Sensitization Test: Local Lymph Node Assay (LLNA) in Mice
Bibliography
A. Lombard et al. - Technical and economic evaluation of products in the early stages of development. Part 2, pp. 153-281, ISTE Publishing Ltd London (2024).
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