Optical devices
Biomedical fluorescence microscopy

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Optical devices


Biomedical fluorescence microscopy

Authors : Léon ESPINOSA, Yves TOURNEUR

Publication date: March 10, 2015 | Lire en français

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2. Optical devices

This chapter covers the principle of epifluorescence microscopy, as well as the necessary ancillary devices such as excitation sources, and technical solutions to the constraints associated with the observation of biological material.

2.1 Full-field microscope

Fluorescence microscopes have come to be known as "widefield" or "full-field" microscopes, to distinguish them from the confocal microscopes that have become widespread since the 1990s.

The fluorescence microscope is based on the classic visible light microscope (optical principles are described in

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